To evaluate the feasibility of DKI in assessment of renal allograft function after transplantation, 13 patients with renal allograft underwent DKI of kidneys，which were divided into two groups according to eGFR. Maps of fractional anisotropy FA, MK, Ka, Kr and MD were generated. There was significant differences in FA, MK, Ka, Kr values of both the cortex and medulla of kidney between two groups. There was significant correlation between eGFR and cortical FA, MK, Ka, Kr, medullary FA, Ka. DKI could be a useful tool in the evaluation of renal function in allograft after transplantation.