Oxygen-enhanced 3D radial UTE MRI (OE-MRI) shows promise as an alternative to hyperpolarized gas MRI for evaluation of ventilation abnormalities. Ten subjects (2 normal, 2 asthmatics and 6 cystic fibrosis) underwent OE-MRI for multiple scans (test/re-test) during visits separated ≤15 days apart. The intra-subject percent signal enhancement (PSE) maps from OE-MRI were compared for median whole-lung PSE, ventilation defect percent (VDP) and spatial agreement between defects. The results suggest good agreement on two global measures, Median PSE and VDP, with low-to-moderate spatial alignment on the inter-visit segmented defects. Improvement of spatial defect repeatability will be a goal of future work.