Diffusion restriction on MRI is an important diagnostic finding in sporadic Jakob-Creutzfeld disease. We performed group-wise quantitative cortical and subcortical grey matter mean diffusivity analysis at 3.0 T and tested feasibility of single-subject diffusivity restriction maps, that, compared to group-wise analysis, can better describe variable patterns of involvement in sJCD. DMN-related regions demonstrated prominent diffusion restriction; thus DMN functional connectivity was evaluated. DMN showed a increased connectivity in sJCD, that based on cross-sectional analysis seems to decrease along with clinical worsening. Resting-state fMRI may be a promising candidate to evaluate involvement in sJCD especially during the preclinical phase.