This study was designed to determine if CBF derived from ASL perfusion imaging could be used to quantitatively evaluate the MVD of brain gliomas on a “point-to-point” basis. The study enrolled 47 patients with treatment-naive brain gliomas who underwent preoperative ASL before stereotactic surgery. We histologically quantified MVD from CD34-stained sections of stereotactic biopsies and co-registered biopsy locations with localized CBF measurements. CBF showed a statistically significant positive correlation with MVD. ASL can be a quantitative and noninvasive perfusion MR method for evaluating the MVD of brain gliomas, and may reflect the microvascularity of gliomas.