Cerebrovascular reactivity (CVR) with hypercapnia challenges, such as a breath-hold (BH) task, has been proposed to indicate areas with neurovascular uncoupling potentials for presurgical fMRI. Previous studies have shown that BH response correlated with resting-state fluctuation of amplitude (RSFA) in healthy adults. This study explores the use of RSFA for indicating sites with neurovascular uncoupling potentials in presurgical fMRI of patients with gliomas. The RSFA with ICA-based denoising approaches was found to perform superior to the traditional approaches. Unlike BH, RS-fMRI is less dependent on patient performance thus can be widely applied in clinical practice.