Mild traumatic brain injury (mTBI) is a prevalent health problem, especially in full-contact sports1, 6. Despite its prevalence, there is still a lack of reliable, unbiased biomarkers of brain injury and recovery following mTBI. Diffusion weighted MRI techniques have gained attention recently in studies of mTBI. Diffusion kurtosis tensor imaging (DKTI) is an extension of the conventional DTI, which estimates non-Gaussianity of bulk diffusion in each voxel. Our studies indicated that DKTI might be potential biomarker to detect subtle changes in brain tissues5. Here, we introduce a workflow to detect sites of brain injury in an individual concussed subject.