Gadoxetate dynamic contrast-enhanced MRI (DCE-MRI) seems promising for non-invasive quantification of liver function. Here we tested the reproducibility of gadoxetate DCE-MRI at four MRI sites using an acute clinical dose of the antibiotic rifampicin. We found significant differences between sites in maximum relative enhancement (RE) in vehicle treated rats. However, highly significant differences in maximum RE between vehicle and rifampicin treated rats was detected at all sites. To our knowledge this is the first multicenter preclinical reproducibility study of an imaging biomarker.