Diabetic neuropathy is a leading cause of diabetic foot ulcer and has been shown to be induced by insulin resistance. Itramyocellular lipid (IMCL) measured by MRS in large skeletal muscles has been shown to correlate with insulin resistance. In this study a generalized Lorentzian approximation is used to estimate the IMCL and EMCL frequency shift as a function of muscle fiber bundle orientation. DTI is used to measure the fiber bundle orientation and provide prior information for IMCL/EMCL peak fitting. 2D-CSI is used to quantify IMCL contents in foot muscle groups of healthy subjects and diabetic patients.