I this study, we investigated which CEST contrast changes are characteristic and dominant for breast malignancies. In 18 female patients, we performed DWI, CEST imaging, and T1-CE MRI. Significant differences in MTRasym between G1 and G3 was found (P= 0.007). Between G1 and G2 (P=0.066) as well as between G2 and G3 no difference were observed (P= 0.089). Correlation test demonstrated a strong positive correlation between mean MTRasym measured in region of its maximum and proliferation factor, Ki-67 (r=0.890, P˂0.001). This preliminary results show that CEST-MRI of the breast has a potential to provide information regarding the tumor histological features.