Diffusion weighted imaging showed the potential to assess bladder cancer. This study aimed to investigate whether readout-segmented EPI can offer better image quality in imaging bladder patients in comparison with single-shot EPI, and to compare quantitative image parameters, derived from RS-EPI with those of SS-EPI. Thirty-five patients were examined using both diffusion techniques. There were significant differences in susceptibility artifacts, lesion detectability, image blurring, CNR and SIR values, except for motion artifacts, SNR and ADC values of the bladder lesions. This study found that the RS-EPI technique provides significant image quality improvement compared with SS-EPI in bladder at 3 Tesla.