The purpose of this study was to investigate if resting state functional MRI is able to reveal brain network changes associated to pregnancy in C57Bl6 mice. 12 mice were scanned before and 3 weeks after pregnancy using a classical resting state fMRI proptocol. Dual regression was performed using these 20 components to find the subject-specific time-series and spatial maps for each network. Significant differences were observed in the striatal, the insula-amygdala and the hippocampal-brainstem networks. Our results reveal that in pregnant C57Bl6 female micethere is reorganization of brain connectivity in specific brain regions and networks.