Abstract #5118

GluCEST MRI: Reproducibility, background contribution and source of glutamate changes in the MPTP mouse model of Parkinson’s disease

Puneet Bagga¹, Stephen Pickup¹, Dan Martinez², Rachelle Crescenzi¹, Ari Borthakur¹, Gaurav Verma¹, Joel Greenberg³, John Detre³, Hari Hariharan¹, and Ravinder Reddy¹

¹Department of Radiology, University of Pennsylvania, Philadelphia, PA, United States, ²Children's Hospital of Philadelphia, Philadelphia, PA, United States, ³Department of Neurology, University of Pennsylvania, Philadelphia, PA, United States

Glutamate Chemical Exchange Saturation Transfer (GluCEST) MRI provides indirect detection of glutamate in vivo by measuring the exchange of glutamate amine protons with bulk water. The GluCEST contrast is potentially contaminated by a contribution of other metabolites exhibiting proton chemical exchange. We evaluated the reproducibility and background contamination to the GluCEST and source of the GluCEST changes in MPTP mouse model. Approximately 28% of GluCEST contrast appears to be derived from sources other than glutamate that are also not detectable by MRS. Glial proliferation caused by neuroinflammation was found to be the cause of elevated glutamate in mice exposed to MPTP.

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