Intracranial conditions are strongly affected by body posture. Apparent diffusion coefficient (ADC) of the brain significantly changes during the cardiac cycle, and this change (ΔADC) reflects intracranial condition. However, the effect of body posture on ΔADC has yet to be confirmed. Therefore, we evaluated the ΔADC and ADC of the brain in upright and supine postures using a multi-posture MRI. ΔADC and ADC of the white matter in the upright posture were significantly higher than those in the supine posture. Multi-posture MRI facilitates the noninvasive evaluation of the effect of gravity on intracranial conditions.