Chemical exchange saturation transfer of GAG (gagCEST) is a quantitative MR technique that is a useful biomarker for assessing GAG content at 7T. However, its utility at 3T remains unclear. In this study, we compare gagCEST asymmetry values of healthy and osteoarthritic subjects scanned at 3T. Comparisons between healthy and OA subjects indicate a significant difference in the average gagCEST signal across the medial and lateral anterior and medial weight-bearing regions of the femoral cartilage. The results of this study suggest that there is potential for use of gagCEST in the study of OA at 3T.