This study assessed changes in ACL T2* metrics throughout the menstrual cycle. In the pre-ovulatory phase, ovulatory case subjects exhibited significant shortening of T2*S and PS in comparison to visit #4 (post-ovulatory phase). Non-ovulatory control subjects displayed no significant changes over time. Results of the current study suggest that there is a shift in bound water (T2*S) within the ACL from pre- to post-ovulatory phases. Shifts in tissue water content have been associated with altered mechanical properties and changes in ligament stiffness may alter proprioceptive sense and contribute to increases in laxity and risk of ACL-injury within the pre-ovulatory phase.