Onset of hyperglycemia and lipotoxicity are associated with reduced β-cell mass and function compromising insulin secretion leading to insulin resistance. It is technically challenging to image pancreas in rodents by conventional imaging approaches. In this study we have utilized GdDOTA-diBPEN to image the pancreas in control and high fat diet (HFD) fed rat model. HFD fed rodent model shows increase in pancreatic fat and functional βcell volume during compensatory phase which is due to the adaptive response to HFD. Longitudinal assessment of pancreatic fat accumulation and βcell mass and function will provide better insights associated with β-cell dysfunction.