Our purpose is to quantificationally evaluate the microvascular permeability and subtle changes in diabetic skeletal muscle by DCE-MRI and texture analysis. Alloxan-induced diabetic rabbits and normal rabbits were studied at fixed time points (0, 4, 8, 12, and 16 weeks). Permeability parameters Ktrans and Kep increased and then showed a downward trend, Ve increased and Vp decreased in diabetic group. Texture parameters based on Ktrans map showed difference between the two groups. We can draw a conclusion that the microvascular permeability of diabetic skeletal muscle increases while the perfusion decreases and texture analysis based on Ktrans map can detect these subtle changes in early stage.