Release of breath hold results in misalignment of the myocardium during cardiac T1 mapping. Several registration methods have been developed to overcome this , however, it has not been established whether the process of respiration effects myocardial T1 when mapped with dynamic MOLLI sequences. 10 healthy volunteers underwent 1.5T MOLLI T1 mapping during both breath hold and free breathing acquisition. Images were registered using synthetic images created via a combined inversion recovery and respiratory signal modulation model, which was verified using Dice Similarity Coefficient. Myocardial T1 was found to be higher in healthy volunteers when acquired during free breathing, when compared to inspiration.