The metabolic alteration in myocardium always accompanied with the structural remodeling. Therefore, it is possible to bridge the imaging markers in CMR with those in 11C-acetate PET imaging. This study investigated the correlation of measurements of interventricular septum between CMR and 11C-acetate in healthy male adults. We found that there was a significant association between ECV measured by quantitative CMR and K1 measured by PET imaging. Our results may indicate a dynamic balance between myocardial blood flow and ECV physiologically. The clinical significance of this relationship needs to be further investigated in patients with cardiac diseases.