Neuroscientific research involving preclinical rodent models often requires the ability to precisely identify anatomical brain regions. This project reports the development of a high-resolution MRI atlas of the Fischer 344 adult rat. The atlas is composed of 98 manually delineated structures through 256 coronal slices. The atlas was developed using 41 adult Fischer 344 rats to generate a co-registered average brain. The template was segmented by intensity contrast in conjunction with the Paxinos and Watson paper atlas. This atlas is intended to be a resource for researchers working with Fischer 344 rats and is provided open-access in MINC2.0 and NIfTI.