In this study, CEST imaging was performed on the substantial nigra, the basal ganglia, and cerebral cortex level to reveal the mechanism each neurodegenerative disease. For this study, patients with Parkinson’s disease, multiple system atrophy, progressive supranuclear palsy, and spinocerebellar degeneration were examined. Region-of-interest analysis was performed in the substantia nigra, red nuclei, lentiform nucleus, and supplementary motor area. As the results, the CEST parameters were significantly different for each of the neurodegenerative diseases. CEST imaging might have the ability to obtain abnormal proteins each of the neurodegenerative diseases.