Lesions in multiple sclerosis (MS) present at various locations throughout the brain. 207 relapsing-remitting MS (RRMS) patients were scanned at 3T. T2w and T1w images were used to segment white matter (WM) hyper- and hypo-intensities, respectively. Using an atlas of WM tracts, the lesion burden was computed for each tract. The dominant lesion load was found in the periventricular regions. The tract percent load is highest in the anterior thalamic radiation, inferior fronto-occipital fasciculus, the forceps major, and forceps minor tracts. The uncinate fasciculus, superior longitudinal fasciculus, and the four cingulum tracts have the lowest lesion loads.