Diffusion kurtosis imaging (DKI) is an emerging technique that provides additional information to delineate tissue microstructures by quantifying the non-Gaussian water molecular diffusion. Although the capability of DKI has been demonstrated, the effects of diffusion gradient parameters on its quantitative metrics, particularly in the clinical setting, have not been fully understood yet. This study aims to investigate the effect of diffusion gradient parameters on diffusion kurtosis tensor calculation and its quantitative metrics. In vivo results show that diffusion gradient duration has incremental influence on DKI quantitative metrics in the clinical setting. Further investigation with more subjects would help to statistically solidify our findings.