Lymph node (LN) involvement is a dynamic process which may be reflected by intravoxel incoherent motion diffusion-weighted imaging (IVIM-DWI). However, there is no such studies so far. Therefore, the potential influence on the diagnosis performance for metastatic LN needs to be investigated. In this longitudinal study on animal models, metastatic LNs evolved in a different pattern from inflammatory LNs pathologically, and IVIM parameters differed between the two groups at particular time points. This indicates that IVIM-DWI can reflect the dynamic pathological changes of LN diseases, and its ability to detect metastatic LN may be affected by the course of disease.