Diffusion-weighted imaging (DWI) based thermometry has a potential to be a non-invasive method of temperature measurement for the deep inside of human brain. Nevertheless, the DWI at lateral ventricle in the brain might be influenced by the pulsation flow of cerebrospinal fluid (CSF), which is motivated by heartbeat. The purpose of this study was to investigate the influence of pulsation flow on brain DWI thermometry for healthy subjects. Comparisons were performed among ΔT at three CSF speed selections (slow vs. fast vs. random). There was no significant difference in ΔT among the CSF speed and volume on healthy subjects.