Abstract #0273

In vivo fluorine-19 MRI of intracellular oximetry response to chimeric antigen receptor T cell therapy against glioma

Fanny Chapelin¹, Wenlian Zhu², Benjamin Leach², Hideho Okada³, and Eric Ahrens²

¹Biomedical Engineering, University of Kentucky, Lexington, KY, United States, ²Radiology, University of California San Diego, La Jolla, CA, United States, ³Neurological Surgery, University of California San Francisco, San Francisco, CA, United States

We explore the temporal dynamics of tumor and T cell intracellular partial pressure of oxygen (pO₂) in a murine flank glioma model receiving chimeric antigen receptor (CAR) T cell therapy. Tumor cells or T cells are intracellularly labeled with perfluorocarbon nanoemulsion prior to injection. ¹⁹F MRI relaxation rate measurements are used to elucidate intracellular pO₂ in vivo. The tumor pO₂ peaks at 3 days post-infusion, commensurate with CAR T cell infiltration and tumor cell killing. Moreover, the absolute ¹⁹F signal scales with tumor burden. Overall, ¹⁹F pO₂ MRI measurements can assay cell-mediated apoptosis and provide insight into effector cell function.

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