Abstract #0630

Non-invasive Detection of M1 Activation in Macrophages using Hyperpolarized 13C MRS of Pyruvate and DHA at 1.47 Tesla

Kai Qiao^1,2, Lydia Le Page^1,2, Celine Taglang^1,2, and Myriam M Chaumeil^1,2

¹Physical Therapy and Rehabilitation Science, University of California, San Francisco, San Francisco, CA, United States, ²Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, CA, United States

The immune system plays an essential role in various diseases, and macrophage activation patterns can vary greatly - impacting intervention. We propose that ¹³C Magnetic Resonance Spectroscopy (MRS) of hyperpolarized [1-¹³C] Pyruvate and [1-¹³C] Dehydroascorbic acid (DHA) can differentiate between non-activated and M1 classically activated macrophages at the clinically-relevant field strength of 1.47T. In M1-activated macrophages we report increased HP Lactate from Pyruvate, and increased HP Ascorbic Acid from DHA compared to Control cells. This study is a first in differentiating between activated and non-activated macrophages with HP probes at this field strength and could become a powerful translational tool.

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