Glucose is the primary fuel for the brain and its metabolism is linked with cerebral function. Isoflurane anesthesia is commonly employed in preclinical MRS but influences functional connectivity. The combination of isoflurane and medetomidine is regularly used in rodent fMRI and show similar functional connectivity as in awake animals. Here we compared the cerebral metabolism of hyperpolarized [2H7,U-13C6]-D-glucose under these two anesthetic conditions. When using the combination, the [1-13C]lactate signal and lactate-to-glucose ratio were more than doubled compared to isoflurane solely, showing that the change of anesthesia had a high impact on cerebral glucose uptake and glycolytic flux.