Methods to improve the early detection of diseases associated with an increased bone fragility are objects of investigation. Single-sided NMR is an appealing approach for medical applications. In this work we propose a new methodology to assess the bone volume fraction (BV/TV) of trabecular bone (TB), without the need of using a reference sample, exploiting the separation between intra- and inter-trabecular 1H signals from quasi-continuous T2 distributions. BV/TV of TB samples estimated using NMR were found in strong agreement with micro-CT estimations. This is promising for the application of single-sided NMR scanners to in-vivo assessing of bone micro-structure.