Single-Shot Echo-Planar Imaging (ssEPI)is highly susceptible to T2* blurring and geometrical distortion. This study aimed to compare the image quality between ssEPI and Readout-Segment Echo-Planar Imaging (rsEPI) for acquiring prostate DWI on 162 patients. Geometrical distortion was ranked on ssEPI and rsEPI using a five-point scale. The geometrical distortion was significantly less observed in rsEPI compared to ssEPI (P<0.01). Geometrical distortion scores of three and higher were observed in 30 individuals in ssEPI, with all having scores < 3 on rsEPI. In conclusion, using rsEPI for DWI acquisition may augment or replace ssEPI on 3T prostate mpMRI.