Spatio-temporal expression control of a heat shock promoter-driven transgene delivered in the kidney by modified mesenchymal stem cells: A feasibility study using MR guided Focused Ultrasound
Delmas Y, Rome C, Couillaud F, Letavernier B, Salomir R, Moonen C, Ripoche J, Combe C, Hauger O, Desmouliere A, Grenier N
University Bordeaux 2
Rat mesenchymal stem cells (MSC) line expressing a luciferase gene under the control of a Heat Shock Protein 70 minimal promoter were injected in the rat renal artery. The kidney was locally heated by MRI-guided focused ultrasound. Induction of expression of the luciferase gene was demonstrated in the heated region using histological examination.