Sensitivity enhanced and localized 13C MRS to study glucose uptake and metabolism in mouse brain
Klomp D, Kan H, Heerschap A, Nabuurs C
Radboud University Medical Center
In vivo 13C MRS measurements with 13C labeled compounds in mice would provide a valuable technique for a better understanding of neurometabolism, especially when feasible in transgenic mice models. Here we present an experimental setup that enables the first 3D localized 13C MRS detection at physiological conditions in mice brain. With polarization transfer techniques and efficient RF coils, a substantial signal enhancement was obtained. The achieved high temporal en spectral resolution of localized 13C MRS opens new opportunities to study neurometabolic fluxes in mouse models.