Detection of lactate dehydrogenase reaction in vivo using carbon-13 magnetization transfer
Xu S, Shen J
National Institute of Mental Health
The carbon-13 magnetization transfer effect due to the rapid exchange between pyruvate and lactate catalyzed by lactate dehydrogenase is reported. The carbonyl carbon of pyruvate at 207.9 ppm was saturated after intravenous infusion of [2-13C]glucose and bicuculline. The results demonstrate that the magnetization transfer effect of lactate dehydrogenase could be used as a novel marker accessible to the noninvasive MRS techniques for studying many physiological and pathological conditions such as cancer.