Assessment of mouse glioma vasculature using SPIO and small molecule contrast agents: sequential implementation of alternate perfusion MRI methodologies
Pike M, Nabors L, Gillespie G, Neumann C, Langford C
University of Alabama at Birmingham
A macromolecular superparamagnetic iron oxide (SPIO) contrast agent, Ferridex, was successfully employed to address first pass extravasation problems in the application of dynamic susceptibility contrast MRI (DSC-MRI) cerebral blood flow (CBF) measurements in models of mouse glioma. Furthermore, in the same imaging session, the approach was combined with dynamic contrast enhanced MRI assessments of vascular permeability using a small molecule contrast agent, in order to obtain a comprehensive assessment of mouse glioma vascularization. The approach lays the groundwork for noninvasive and highly diagnostic investigations of anti-angiogenesis strategies in mouse models of malignant glioma.