Noninvasive in vivo redox status study by fast acquisition proton electron double resonance imaging
Li H, Xi X, Cardounel A, Sun Z, Petryakov S, Deng Y, Kuppusamy P, Zweier J
Davis Heart & Lung Research Institute, Ohio State University
In order to map and monitor fast metabolism state of free radicals in vivo, temporal and spatial resolution are very critical. A suitable fast acquisition proton electron double resonance imaging sequence based on fast spin echo pulse sequence was developed and first time applied for noninvasive in vivo redox status study of mice. Temporal resolution (~ 3.4 s) and spatial resolution (~ 1.2 mm) were achieved. Both control and treated mice were studied by using TEMPONE nitroxide as probe. It was demonstrated that Cardax treatment significantly altered the tissue redox status of the mice heart and reduced the oxidant levels.