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Abstract #1983

Insulin Release and Energetic Changes in INS-1 Cell Bioreactors in Response to Glucose Challenge

Bieber B, Olson L, Krohn K, Wiseman R
Michigan State University

The functional coupling between mitochondria and insulin release was examined in a beta cell model using phosphorus NMR spectroscopy of small cell bioreactors. Results show that a first order change in cytosolic ADP is highly correlated with insulin release and that ATP remained unchanged. This suggests that ADP homeostasis is a controlled variable and offers insight into the role of both adenylates and of mitochondrial energetics in insulin secretion in this cell line.