Multiexponential T2 Analysis in Cartilage Degraded Using Different Enzymatic Protocols

David A. Reiter¹, Ligaya Roque¹, Richard G. Spencer¹

We sought to improve the specificity for cartilage matrix degradation through multiexponential analysis of T₂ relaxation, which can delineate macromolecular compartments with varying water fraction and mobility. Multiexponential T₂ analysis of control cartilage and of cartilage subjected to three enzymatic degradation protocols showed distinct patterns, consistent with the known actions of the degradative enzymes used. In contrast, while monoexponential T₂ values increased with degradation, there was no enzyme-specificity in this increase. Therefore, multiexponential T₂ analysis demonstrates greatly improved specificity over standard monoexponential T₂ analysis to changes in cartilage matrix, indicating the diagnostic potential of such analysis for detecting cartilage disease.