Tomoyasu Mani1,2, Osamu Togao1, Todd C. Soesbe1, Masaya Takahashi1, Allan Dean Sherry1,2
1Advanced Imaging Research Center, University of Texas Southwestern Medical Center at Dallas, Dallas, TX, USA; 2Department of Chemistry, University of Texas at Dallas, Richardson, TX, USA
Chemical exchange saturation transfer (CEST) imaging has attracted considerable attention. Ln(III)-based exogenous CEST agents (PARACEST) can be used to enhance the sensitivity of CEST imaging. As part of a program to develop more effective agents, a newly designed Eu(III)-DOTA-tetraamide complex was applied in vivo. The CEST effect from this agent were somewhat smaller when the agent was dissolved in blood plasma at body temperatures (35-39 C) compared to when it was dissolved in pure water. No differences in CEST were observed at lower temperatures (20-27 C). CEST contrast in liver and kidney of mouse (20%) were successfully observed using low presaturation pulse (7T) to avoid competitive inherent MT effects.