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Abstract #0238

Simplified 13C Metabolic Modeling for Simplified Measurements of Cerebral TCA Cycle Rate in Vivo

Julien Valette1, Fawzi Boumezbeur1, Vincent Lebon1,2

1CEA-NeuroSpin, Gif-sur-Yvette, France; 2CEA-MIRCen, Fontenay-aux-Roses, France

13C NMR spectroscopy is a unique tool to measure cerebral TCA cycle rate in vivo. The measurement relies on metabolic modeling of glutamate C3 and C4 enrichment time-courses during a 13C-glucose i.v. infusion. Classical metabolic models require as additional input functions the plasma glucose and 13C-glucose time-courses, as well as the knowledge of Michaelis-Menten kinetics parameters governing passage through the blood brain barrier. It is shown in the present work that metabolic modeling can be simplified in a manner that these additional inputs are not required anymore, significantly simplifying the measurement of cerebral TCA cycle rate in vivo.