Abstract #0455

Comparison of DTI Fiber Tracks with Light Microscopy of Myelinated Fibers

Ann Sunah Choe^1,2, Xin Hong^1,2, Daniel Christopher Colvin^1,2, Iwona Stepniewska³, Zhaohua Ding^1,2, Adam W. Anderson^1,2

¹Vanderbilt University Institute of Imaging Science, Vanderbilt University, Nashville, TN, USA; ²Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA; ³Department of Psychology, Vanderbilt University, Nashville, TN, USA

Fibers tracked using Diffusion Tensor Imaging (DTI) are directly compared with myelin stained fibers on a microscopic level. Gold standard measurements of fiber orientation and spread from micrographs enabled us to investigate the challenges of DTI fiber tracking in brain tissue. Limitations due to partial volume averaging and image noise are readily observed.