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Abstract #0873

Intracellular MRI Contrast by SPIOs and Dy Chelates at 11.75 and 21.1 T

Jens Thorvald Rosenberg1,2, Joshua M. Kogot3, Chris Ridel3, Geoffery F. Strouse3, Samuel C. Grant1,2

1The National High Magnetic Field Laboratory, Tallahassee, FL, USA; 2Chemical and Biomedical Engineering, Florida State University, Tallahassee, FL, USA; 3Chemistry and Biochemistry, Florida State University, Tallahassee, FL, USA

We have compared intracellular SPIOs in murine microglia cells (Bv-2) at two field strengths, 11.75 and 21.1 T. Cells incubated with SPIOs were immobilized in 1% agarose layers for imaging. T1, T2 and T2* were assessed. Two experimental variables were tested, agent dosing and cell count. Agent dosing showed no difference between field strengths while variations in the cell count did display field-related effects. Dy-DOTA and Dy-DTPA were also evaluated. Dy-DOTA showed increased uptake, possibly due to the more positively charged DOTA molecule. These findings impact the choice of contrast agent for high field studies of implanted cell lines.