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Abstract #0918

In Vivo Monitoring of Transplanted Human Embryonic Stem Cell-Derived Oligodendroglial Progenitors in a Mouse Model of Multiple Sclerosis

Heechul Kim1,2, Candace Kerr3,4, Naser Muja1,2, Piotr Walczak1,2, Jeff W.M. Bulte1,2

1Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, USA; 2Cellular Imaging Section, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD, USA; 3Dept. of Gynecology and Obstetrics, The Johns Hopkins University School of Medicine, Baltimore, MD, USA; 4Stem Cell Biology Program, , Institute for Cell Engineering , The Johns Hopkins University School of Medicine, Baltimore, MD, USA


Feridex-labeled hESC-derived oligodendroglial progenitors were transplanted and tracked in a mouse model of multiple sclerosis. Feridex-labeling did not impair the therapeutic benefit induced by transplanted cells. At day 1 post-tx, hypointense MRI signals were detected mainly in the ventricle and subventricular zones. These signals persisted on days 5, 15, and 30 post-tx.The hypointense areas within the ventricle were significantly decreased at day 30 post-tx as compared to days 1, 5, and 15. Based on the above results, we postulate that cell migration mainly occurs through the ventricular system to the parenchyma during days 15-30 post-tx.

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