Abstract #0922

Efficient Labeling of Dendritic Cells with a Clinical Applicable Perfluorpolyether Compound for Quantitative 19F MRI Tracking in Cancer Patients

Fernando Jos Bonetto¹, Mangala Srinivas¹, Arend Heerschap², Robbie Mailliard³, Eric T. Ahrens⁴, Carl Figdor⁵, Jolanda de Vries⁶

¹Tumor Immunology, Nijmegen Centre for Molecular Life Science, Radboud University Nijmegen medical Centre, Nijmegen, Gelderland, Netherlands; ²Radiology, Radboud University Nijmegen Medical Center, Nijmegen, Gelderland, Netherlands; ³Celsense Inc., Pittsburgh, USA; ⁴Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA; ⁵Nijmegen Centre for Molecular Life Science, Radboud University Nijmegen Medical Center, Nijmegen, Gelderland, Netherlands; ⁶Tumor Immunology, Nijmegen Centre for Molecular Life Science, Radboud University Nijmegen Medicla Centre, Nijmegen, Gelderland, Netherlands

Tracking of cells using contrast agents with magnetic resonance imaging provides detailed anatomical information but it lacks quantitative information. A new perfluoropolyether nanoemulsion was studied for labeling of dendritic cells for 19F MRI. Our results demonstrate that the compound is taken up by the cells and does not hamper cell function ( viability, maturation and mRNA uptake).