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Abstract #0929

Serial Monitoring and Quantification of Endogenous Neuroblast Migration Rates by Cellular MRI

Dorit Granot1, Dustin Scheinost1, Xenophon Papademetris1,2, Erik M. Shapiro1,2

1Department of Diagnostic Radiology, Yale University School of Medicine, New Haven, CT, USA; 2Department of Biomedical Engineering, Yale University, New Haven, CT, USA

Here we quantified the rate of migration of native neuroblasts into the olfactory bulb by MRI. Neuroblasts were labeled in-vivo by direct injection of MPIOs into the ventricle. Following labeling, we measured a linear accumulation of newly arriving cells in the OB. At day 1, cells were not found in the OB. By day 3, cells were observed entering the central portion of the OB. At day 8, many cells were detected in the central portion of the OB and in the outer edges. MRI detection of migration plateaued at week 1, indicating that the MPIO injection was a bolus.