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Abstract #1015

1H MAS NMR Spectroscopy of Metabolites and Lipids During Cell Growth Arrest Induced by Cisplatin in Cultured Rat Glioma BT4C Cells.

Ladan Mirbahai1, Martin Wilson2, Christopher S. Shaw, Carmel McConville2, Roger D.G Malcomson3, Risto A. Kauppinen4, Andrew C. Peet2

1 School of Sport and Exercise Sciences, University of Birmingham, Birmingham, West midlands, UK; 2School of Cancer Sciences, University of Birmingham, Birmingham, UK; 3Department of Histopathology, Birmingham Childern's Hospital NHS Foundation Trust, Birmingham, UK; 4Dartmouth Medical School, Hanover, NH, USA

The possibility of using the detected alterations in lipid and metabolite profiles as early biomarkers of cisplatin treatment, prior to appearance of morphological characteristics of apoptosis is suggested in this study. Lipid and metabolite alterations were investigated in cisplatin treated rat glioma cells by 1H MAS NMR spectroscopy. The presence of intracellular lipid vesicles was examined by TEM and Nile red staining. To determine cell viability status TB, H&E staining, and DAPI were used. The growth cycle stage was determined by flow cytometry. Lipid and metabolite alterations were detected at early stages after treatment when most cells appeared morphologically viable.