Meeting Banner
Abstract #1016

1H MRS Detected Mobile Lipids in Rodent Gliomas: Correlation with EPR Determined Tumour Oxygenation

S K. Hekmatyar1, Timo Liimatainen2, Neil Jerome1, Nadeem Khan3, Harold M. Swartz3, Risto A. Kauppinen1

1Biomedical NMR Research Center, Dartmouth Medical School, Hanover, NH, USA; 2Center for Magnetic Resonance Research, University of Minnesota,, Minneapolis, MN, USA; 3EPR Center, Radiology, Hanover, NH, USA

1H MR Spectroscopy detected lipids serve as biomarkers both for brain tumour classification and treatment monitoring. In the present study, two malignant rat glioma (9L and F98) models were used to examine tumour oxygenation (ptO2) by electron paramagnetic resonance (EPR) and 1H MR spectroscopic imaging to map the saturated lipids (1.3ppm) and lactate. Lipid 1.3ppm/creatine ratio was 2.5-times higher in F98 than in 9L glioma, but differences in lactate/Cr ratios were not detected. EPR showed ptO2 of 5.90.8 in F98 and 34.93.3 mm Hg in 9L. These data indicate association of 1H MRS lipids with hypoxic tumour microenvironment.