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Abstract #1018

High Resolution 13C HR-MAS Spectroscopy Analysis of Different Brain Regions from Rats Bearing C6 Implanted Gliomas

Valeria Righi1,2, Pilar Lopez-Larrubia2, Luisa Schenetti3, Vitaliano Tugnoli4, Maria-Luisa Garcia-Martin2,5, Sebastian Cerdan2

1Department of Surgery, NMR Surgical Laboratory,MGH Harvard Medical School, Boston, Massachussetts, USA; 2Instituto de Investigacion Biomedicas "Alberto Sols", Madrid, Spain; 3Departement of Chemistry, Universit di Modena, Modena, Italy; 4Departement of Biochemistry, Universit di Bologna, Bologna, Italy; 5RM Ntra. Sra. del Rosario, Madrid, Spain

We demonstrated that 13C High Resolution Magic Angle Spinning (HR-MAS) NMR is a useful tool to detect and quantify the 13C in cerebral metabolism and opening the way to investigate cerebral tissue heterogeneity within the microliter range. Recently, by using HR-MAS high quality 13C spectra from small tissue biopsies (10 mg) were obtained. Here, we report for the first time to our knowledge, a 13C HR MAS study of normal and diseased brain regions of rats bearing C6 gliomas implanted. The expression of the genes involved in the glycolytic metabolism was investigated in brain biopsies from the same cerebral regions.