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Abstract #1909

P31 NMR Demonstrates Dysfunction Through Mitochondrial Uncoupling in Skeletal Muscle After Burn Injury

Dionyssios Mintzopoulos1, Meenu Kesawarni1,2, Laurence G. Rahme3, Ronald G. Tompkins4, A Aria Tzika1

1NMR Surgical Laboratory, MGH & Shriners Hospitals, Harvard Medical School, Boston, MA, USA; 2Molecular Surgery Laboratory, MGH & Shriners Hospitals, Harvard Medical School, Boston, MA, USA; 3Molecular Surgical Laboratory, MGH & Shriners Hospitals, Harvard Medical School, Boston, MA, USA; 4Surgery, MGH & Shriners Hospitals, Harvard Medical School, Boston, MA, USA


We employed in vivo P31 NMR on intact uncoupling protein 3 (UCP3) knock-out and wild-type (control) mice, following 30% total burned surface area burn to assess the ATP synthesis rate in skeletal muscle. Our results revealed that the rate of ATP synthesis in UCP3 mice is significantly reduced as compared to controls. These findings implicate mitochondrial uncoupling in skeletal muscle following burn injury.