Bhavana Shantilal Solanky1, Gina J. Sanchez-Canon1, Jeremy F L Cobbold2, Simon D. Taylor-Robinson3, Jimmy D. Bell1, Julie C. Holder4, I Jane Cox2, Po-Wah So5
1Metabolic and Molecular Imaging Group, Clinical Sciences Centre, Hammersmith Hospital, Imperial College London, London, UK; 2Imaging Sciences Department, Clinical Sciences Centre, Hammersmith Hospital, Imperial College London, London, UK; 3Division of Medicine, Faculty of Medicine, Hammersmith Hospital, Imperial College London, London, UK; 4Safety Assessment, GlaxoSmithKline Pharmaceuticals, Ware, UK; 5Biological Imaging Group, Clinical Sciences Centre, Hammersmith Hospital, Imperial College London, London, UK
31P MRS and 1H MAS MRS was used to study ANIT-induced hepatobiliary dysfunction in rats. Three groups of animals were fed a diet containing 0.05%, 0.04% and 0.025% ANIT for 14 days. Biliary damage was confirmed in all groups and was characterised by increased PDE due to cell degeneration, and increased PC+GPC in the higher dose groups. Hepatocellular damage was only confirmed in the 0.05% group, this corresponded to increases in PME due increased mitotic activity in the peribiliary region and increased total choline. This work supports the use of 31P MRS and 1H MAS MRS to study hepatobiliary disease.
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