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Abstract #2164

Prolongation of Pancreatic Islet Transplant Viability: Comparison of Preservation Methods by Phosphorus Magnetic Resonance Spectroscopy

Alan Bainbridge1, Aditya Agrawal2, Stephen Powis2, Ernest Cady3, Barry Fuller2, Brian Davidson2

1Medical Physics & Bio-Engineering, UCL Hospitals NHS Foundation Trust, London , UK; 2University Department of Surgery, Royal Free and University College of London Medical School, London, UK; 3Medical Physics & Bio-Engineering, UCL Hospitals NHS Foundation Trust, London, UK

Minimizing pancreatic cold preservation injury is critical for successful islet of Langerhans transplantation. In the two-layer method (TLM) of organ preservation, developed to minimize the detrimental effect of cold ischaemia, the pancreas is maintained at the interface between perfluorocarbon and a conventional preservation solution. This study aimed to develop an experimental model for dynamic assessment of pancreatic energy metabolism throughout preservation using phosphorus (31P) magnetic resonance spectroscopy (MRS). TLM prolongs rat pancreas viability better than Marshalls solution alone. Continuous preservation-medium oxygenation increases the duration of this beneficial effect.