Abstract #2164

Prolongation of Pancreatic Islet Transplant Viability: Comparison of Preservation Methods by Phosphorus Magnetic Resonance Spectroscopy

Alan Bainbridge¹, Aditya Agrawal², Stephen Powis², Ernest Cady³, Barry Fuller², Brian Davidson²

¹Medical Physics & Bio-Engineering, UCL Hospitals NHS Foundation Trust, London , UK; ²University Department of Surgery, Royal Free and University College of London Medical School, London, UK; ³Medical Physics & Bio-Engineering, UCL Hospitals NHS Foundation Trust, London, UK

Minimizing pancreatic cold preservation injury is critical for successful islet of Langerhans transplantation. In the two-layer method (TLM) of organ preservation, developed to minimize the detrimental effect of cold ischaemia, the pancreas is maintained at the interface between perfluorocarbon and a conventional preservation solution. This study aimed to develop an experimental model for dynamic assessment of pancreatic energy metabolism throughout preservation using phosphorus (31P) magnetic resonance spectroscopy (MRS). TLM prolongs rat pancreas viability better than Marshalls solution alone. Continuous preservation-medium oxygenation increases the duration of this beneficial effect.